Tritium - Labeled Compounds II . General - Purpose Appar - and Procedures Use of Tritium atus , and Lithium for the Preparation , Analysis , Oxide and Tritium - Labeled Borohydride

A general-purpose manifold is descd bed, which is useful for numerous proced ures involving trit ium gas and tritium-labeled mate rials. M ethods a re given in detail fOI' (a) converting t ritium gas to tritium oxide, (b) preparing tritium-labeled li thium borohy dride, and (c) conducting a variety of r eactions in a closed system. Auxilia ry equipment is shown, including water traps, reaction flasks, and apparatus for preparing solu tions an d makin g filtrat ions in closed systems. Methods a rc presented fOI' assaying tritium-labeled \\"ater by (a) dissolvin g it in a phosphoric an hydride-sulfuric acid sollltion and counting in a windowl ess, gas-flow, proportional counter, 0 1' (b) convert in g it to hydrogen-t and assaying it in an ionization chamber. The use of tritium-labeled lithium bo rohydride is i llustrated by t he preparation of D-galactitol-l-t from D-galactose. Subseq ucnt papers will desc ribe t he use of this apparat us in the synt hes i s of tri t iu m-Iabeled e"l.rbollydrates. The detail ed descrip tion of t echniq ues an d apparatus sho uld be helpful to ot hers who are illte rested in us ing t r itium,